Composite

Part:BBa_K3809009:Design

Designed by: Francisco Javier Castañeda Villagran   Group: iGEM21_TecCEM   (2021-10-07)


mRFP + oxyR homology regions + FLP recognition sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for E. coli.


Source

The sequence for the promoter comes from BBa_J23100, the RBS comes from BBa_B0030, the terminator comes from BBa_B0010, the spacer comes from BBa_B0040. The homology region sequence comes from Escherichia coli str. K-12 substr. MG1655, complete genome; with an NCBI Reference Sequence NC_000913.3. The reporter sequence was synthetized based on part BBa_J23100.

References